ABSTRACT
In The history of Sexuality, Foucault described the bipolar form of biopower that appeared in modern West: the disciplining power for the individual body and the "biopolitics" targeted the population, and used it to analyze the pandemic that appeared in Europe in the 17th century. By associating Foucault’s biopower with authoritarianism, Agamben created a new version of biopolitics, used it to understand the COVID-19 in Italy, and pointed out that it was the state sovereignty manipulating individual life under "exceptional conditions" to create "naked life". Facing the pandemic, Agamben’s biopolitics obviously cannot explain the rationality of public health interventions. Foucault avoided this problem to some extent, while his liberal biopolitics under the framework of liberalism may still lead to violence against pandemic victim and animal. In view of the limitations of biopolitics in understanding the pandemic, this paper turned the perspective to public health ethics, and tried to think about the coordination between individual and collective interests on the basis of the common good.
ABSTRACT
cry1Ah1, one of holo-type cry genes, cloned in this laboratory from Bacillus thuringiensis strain has been patented in China, and it encoded a protein with strong insecticidal activity against certain lepidopteran insect pests, such as Chilo suppressalis. cry1Ah1 gene is exhibiting good application prospects. In order to improve the expression level of cry1Ah1 gene in rice, and investigate the effect of codon usage preference of gene expression, we designed five different optimized schemes for cry1Ah1 insecticidal critical fragment in accordance with bias of rice codon, to improve G+C content, removed the shear signal and unstable factors. Optimized cry1Ah1 genes were transformed into Escherichia coli Rosetta (DE3) respectively, and 65 kDa polypeptides was expressed normally in inclusion body separately. All of these expressed polypeptides showed insecticidal activity against 2nd-instar larvae of Plutella xylostella and neonate of Chilo suppressalis. After transformation with modified cry1Ah1 genes into Var nippobare, the transgenic rice seedlings were detected by PCR, the positive rate containing target gene was more than 87%. Afterwards, the results of real-time RT-PCR and ELISA assay indicated that the highest expression level of five modified cry1Ah1 genes was that using the highest frequent codons. Average expression amount of Cry1Ah1 polypeptides was 0.104% of total soluble proteins from the positive transgenic rice.
Subject(s)
Animals , Bacillus thuringiensis , Genetics , Metabolism , Bacterial Proteins , Genetics , Cloning, Molecular , Codon , Genetics , Endotoxins , Genetics , Hemolysin Proteins , Genetics , Insecticides , Lepidoptera , Oryza , Genetics , Pest Control, Biological , Methods , Plants, Genetically Modified , Genetics , Recombinant Proteins , GeneticsABSTRACT
The yeast one hybrid system is the new type system deriving from the yeast two hybrid system to study the interaction between DNA and protein.The principle and the technical line of this system was briefly introduced,and the progress of this system on the research of plant transcription factor gene resistant to osmotic stress was particularly summarized,such as cloning the transcription factor genes of osmosis resistance type;confirming the interaction between known DNA and protein;making sure the localization of the confirmed DNA binding domain with the interaction;also validating the transcription activation of the transcription factor gene.Furthermore,the problems existing in this system on the research of plant transcription factor gene resistant to osmotic stress was analyzed,and the development prospect according to the research was forecasted.
ABSTRACT
@#: Objective To observe the effect of femoral retrograde interlocking intramedullary nails on distal femoral fractures.Methods21 patients with distal femoral fractures who were treated with open reposition and femoral retrograde interlocking intramedullary nails were followed-up.Results18 patients were followed up.All the cases obtained bony union and good functional recovety.No knee pain,lameness or stiff knee joint was found.ConclusionFemoral retrograde interlocking intraInedullary nails result in firm fixation,fast functional recovery, and less complications in treatment of distal femoral fractures.